Objective: Basketball is a sport characterized by open skills, where actions are unpredictable and dynamics are constantly shifting, leading professional players to experience varying levels of physical and mental fatigue. This study aimed to explore and compare how mindfulness and music training affect physical and mental fatigue in elite basketball players. Method: In this quasi-experimental research, 27 elite basketball players were randomly assigned to either a mindfulness-based training group, a music-based training group, or a control group. Blood samples were taken under fasting conditions to assess the study variables before and after the intervention. Data analysis involved one-way analysis of variance (ACNOVA), paired t-tests with a significance threshold (P ≤ 0.05), and the Bonferroni post hoc test to identify intra-group and inter-group differences. SPSS version 27 was utilized for these analyses.Results: When comparing the groups, there was no significant difference in the impact of mindfulness and music on physical fatigue at a significance level (P ≤ 0.05). Similarly, the effect of mindfulness and music on changes in inflammatory markers such as lactate dehydrogenase, creatine kinase, and C-reactive protein was not significantly different at the same significance level. However, the effect of mindfulness and music on mental fatigue showed a significant difference at a significance level (P ≤ 0.05), indicating that mindfulness significantly reduces psychological fatigue.Conclusions: The findings of this study revealed no significant difference between the effectiveness of mindfulness-based training and music in terms of physical fatigue and changes in serum levels of lactate dehydrogenase, creatine kinase, and C-reactive protein. However, in the area of mental fatigue, mindfulness-based training demonstrated a significant reduction in mental fatigue in the post-test.